Journal: EMBO molecular medicine
Article Title: FABP4 as a therapeutic host target controlling SARS-CoV-2 infection.
doi: 10.1038/s44321-024-00188-x
Figure Lengend Snippet: Figure 4. Inhibition of FABP4 in bronchial epithelial cells disrupts virus replication organelles.
Article Snippet: ATCC CRL-3386 Fabp4 knockout 3T3-L1 In house Vero E6 cells ATCC CRL-1586 HBE135-E6E7 ATCC CRL-2741 MRC5 lung fibroblasts ATCC CCL-171 HEK293 cells ATCC CRL-1573 Airway epithelium organoids MATTEK AIR-100, AIR-112 SARS-CoV-2 strain USAWA1/2020 BEI resources NR-52281 SARS-CoV-2 strain Ank1 GenBank MT478019 SARS-CoV-2 strain AnkDlt1 GenBank OM295705 SARS-CoV-2 strain Ankomicron GenBank OR529199 Reagent/Resource Reference or Source Identifier or Catalog Number SARS-CoV-2 strain AnkEris Partial sequencing SARS-CoV-2 strain SARSCoV-2 BetaCoV VirNext IDF0571 Human β-coronavirus OC43 ATCC VR-1558 Syrian Hamsters (Mesocricetus auratus) Janvier laboratorie RjHan:AURA Recombinant DNA lentiCRISPRv2 Addgene 52961 Antibodies Rabbit anti-SARS-CoV-2 nucleocapsid Abcam ab271180 Mouse anti-SARS-CoV-2 nucleocapsid Cell Signaling Technology 33717 Rabbit anti-β-actin Abcam ab8224 Rabbit anti-GAPDH Cell Signaling Technology 5174S Mouse anti-FABP4 Dana Fabor Antibody Core 351.4.2E12.H1.F12 Mouse anti-FABP4 Dana Fabor Antibody Core 351.4.5E1.H3 Rabbit anti-FABP4 Abcam ab216708 Rabbit anti-FABP4 Abcam 13979 Goat anti-FABP4 Novus AF1443 Rabbit anti-FABP4 Sigma HPA002188 Mour anti-double stranded RNA J2 Exalpha 10010500 Rabbit anti-calnexin Cell Signaling Technology 2679 Anti-mouse secondary antibody Cell Signaling Technology 4410 Anti-rabbit secondary antibody ThermoFisher Scientific A-11037 Anti-rabbit HRPconjugated antibody Abcam ab64264 Anti-SARS-CoV-2 nucleocapsid GeneTex GTX635686 Oligonucleotides and other sequence-based reagents FABP4 targeting shRNA Origene TL313105 Scrambled shRNA Origene TR30021 SARS-CoV-2 nucleocapsid ThermoFisher Scientific Vi07918637_s1 SARS-CoV2 ORF1ab ThermoFisher Scientific Vi07921935_s1 Human FABP4 ThermoFisher Scientific Hs01086177_m1 Human β-actin ThermoFisher Scientific Hs01060665_g1 Chemicals, Enzymes and other reagents DMEM/high glucose media ThermoFisher Scientific 11965118 FBS Atlanta Biologicals S11550 Penicillin streptomycin Lonza 17-603E Human insulin Sigma-Aldrich I9278 Biotin Sigma-Aldrich B4639 Panthothenate Sigma-Aldrich P-5155 12 EMBO Molecular Medicine © The Author(s) D ow nloaded from https://w w w .em bopress.org on February 20, 2025 from IP 150.162.77.29.
Techniques: Inhibition, Virus